II This procedure is used to prepare 5% and% FBS supplemented media for tissue cultureDescription culture media used. Other Complex Addenda (organic supplements) Plant Growth Regulators I. OBJECTIVES. To become familiar with the necessary nutritional and environmental factors for culturing microorganisms in the laboratory. Approved: M. Wicha. Composition of Culture Medium. Plant tissue and cell culture media are generally made up of some or all of the following components: macronutrients, micron